THE X. WILLIAM YANG RESEARCH GROUP @ UCLA

1. UCLA Mental Retardation Center BAC Transgenic Facility (under construction)

2. Reviews on BAC transgenesis and applications:

• Yang XW, Gong S. An Overview on Generation of BAC Transgenic Mice for Neuroscience Research. Current Protocols in Neuorscience (Link).

• Gong S, Yang XW. Modification of Bacterial Artificial Chromosomes (BACs) and Preparation of Intact BAC DNA for Generation of Transgenic Mice. Current Protocols in Neuorscience (Link).
  
  Heintz N. BAC to the future: the use of bac transgenic mice for neuroscience research. Nat Rev Neurosci. 2001 Dec;2(12):861-70. (PubMed)

• Copeland NG, Jenkins NA, Court DL. Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet. 2001 Oct;2(10):769-79.(PubMed)

3. Databases to locate BACs:

• UCSC

• Sanger Center

• NCBI/NIH

• Celera genomics

4. Ordering BACs:

• BAC/PAC Resource

• Invitrogen

• Open Biosystems

• Sanger Center (129 BACs)

5. RecA-Based Methods to modify Bacterial Artificial Chromosome by homologous recombination

• Basic Procedure: Protocol

• High Speed BAC transgenesis: Protocol
  

• Detailed Protocols

  • Gong and Yang, Current Protocols, 2005.

  • Gong et al, Genome Research

6. Methods to purify BAC for pronuclear injection to develop transgenic mice:

• Gong and Yang, 2005

• Michigan;

• Biotechnique

7. Large Scale BAC transgenic studies:

• GENSAT

• Velocigene